Growth of cutaneous propionibacteria on synthetic medium; growth yields and exoenzyme production
Holland, K.T.; Greenman, J.; Cunliffe, W.J.
Journal of Applied Bacteriology 47(3): 383-394
1979
ISSN/ISBN: 0021-8847 PMID: 541303 Document Number: 140718
A synthetic medium containing only low MW components was developed to grow Propionibacterium acnes, P. avidum and P. granulosum. The medium supported the growth of 30/32 typed strains, and when solidified with agar supported the growth of these bacteria isolated directly from the skin. The mean overall efficiency of isolation on the synthetic medium was 57% that of reinforced clostridial medium (RCM). Batch culture experiments with glucose, fructose, glycerol or arginine in the medium showed that the concentrations and the type of C energy sources used could effect growth and exocellular enzyme production by these organisms.