Relative sensitivity of various cell cultures to the thermostable exotoxin of B. thuringiensis
Laurent, P.
Revue Canadienne de Biologie 37(2): 127-130
1978
ISSN/ISBN: 0035-0915 PMID: 100834 Document Number: 138728
The "thermostable" B. thuringiensis exotoxin is active on cell cultures of Mammals "in vitro", except on the KB strain from a human tumor. The primary cultures are the most sensitive: first, with monkey kidney cells, the growth is inhibited by 0.1 mg of toxin per ml; next, the young rabbit kidney cells react to 0.25 mg of toxin per ml. The established lines of cells come last: human diploid cells (Lyon 4) and heteroploid cells (BHK21C13), with the same active dose of 1 mg of toxin per ml. No protection is obtained by adding ATP to monkey kidney cells at the same time as the exotoxin.