Novel Recombinant Sapovirus in Bangladesh

Dey, S.K.; Mizuguchi, M.; Okitsu, S.; Ushijima, H.

Clinical Laboratory 57(1-2): 91-94

2011


ISSN/ISBN: 1433-6510
PMID: 21391471
Document Number: 13774
Background: Recombination of RNA viruses plays an important part in molecular epidemiological study, virus evolution, vaccine design, and viral control programs. Sapovirus, a member of the family Caliciviridae , is one of the major causative agents of viral gastroenteritis affecting all age groups. Methods: Sapovirus capsid and polymerase regions were amplified by PCR using specific primers. PCR products were sequenced directly and sequence analysis was performed using CLUSTAL X, SimPlot, and MEGA 4 software package. Results: Based on the genetic analysis, a novel, naturally occurring recombinant sapovirus strain was identified in Bangladesh. Breakpoint analysis of the recombinant sapovirus showed that the recombination site was at the open reading frame ORF1/ORF2 overlap. Conclusions: We described the genetic characterization of a novel, naturally occurring recombinant sapovirus and provided the first evidence of recombination in sapovirus in Bangladesh.

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Novel Recombinant Sapovirus in Bangladesh