Strongyloides stercoralis recombinant NIE antigen shares epitope with recombinant Ves v 5 and Pol a 5 allergens of insects
Ravi, V.; King, T.Piao.; Andersen, J.F.; Nutman, T.B.; Neva, F.A.
American Journal of Tropical Medicine and Hygiene 72(5): 549-553
2005
ISSN/ISBN: 0002-9637 PMID: 15891128 Document Number: 590089
A new recombinant protein (NIE) for immunodiagnosis Of human Strongyloides infection has 13% to 18% amino acid identify with antigen 5 insect venom allergen. but the C-terminal segment of NIE showed highest identify with Ves v 5 (yellow jacket) and Pol a 5 (paper wasp). A rabbit polyclonal anti-NIE antibody identified a single band of NIE antigen as well as hands of Pol a 5 and Ves v 5 antigens, and mouse anti-Pol a 5 and anti-Ves v 5 sera reacted with recombinant NIE antigen by Western blot. A cyanogen bromide-digested C-terminal fragment of NIE was reactive with mouse anti-Ves v 5 and Pol a 5 antibodies as well as with rabbit anti-NIE serum. Although IgE and IgG antibodies from pooled sera from Strongyloides-infected patients reacted with Pol a 5 and Ves v 5 recombinant antigens on immunoblots, neither antigen inhibited human IgG reaction with NIE antigen in a competitive enzyme-linked immunosorbent assay.