Preliminary studies on manganese free radical in vasectomy
Chinoy, N.J.; Seethalakshmi, L.
Indian Journal of Experimental Biology 16(12): 1318-1320
1978
ISSN/ISBN: 0019-5189 PMID: 750426 Document Number: 137598
Electron spin resonance spectrometric studies of Mn2+ free radical in the cauda epieymal sperm suspensions of normal, vasectomized and vasectomized plus ascorbic acid treated rats were conducted. In each case, the characteristic 6 hyperfine line-spectrum of g=2.003 at a magnetic field of 3390 gauss was obtained. Healthy adult male albino rats of the Holtzman strain were used for the experiments. Analysis of the spectra showed that the spin concentrations of free radical of the experimental groups were not significantly different from those of the control. Bilateral vasectomy was performed by the standard technique used in human beings. The rats were divided into 3 groups: normal intact control; bilaterally vasectomized for 10 days; and, bilaterally vasectomized and given ascorbic acid orally (100 mg/day/animal) for 10 days. The sperm suspensions were prepared in a known volume of .9% saline solution. Equal amounts of 10% PCA were added to the sperm suspension. Considering the importance of Mn2+ in various reproductive functions, it is evident that vasectomy does not affect the utilization of Mn2+ by epididymis.