A further contribution on nucleoli of human lymphocytes
Raska, I.; Smetana, K.
Folia Haematologica 105(2): 200-215
1978
ISSN/ISBN: 0323-4347 PMID: 79525 Document Number: 134541
Cultured human lymphocytes were investigated by means of light microscopic and EM procedures to provide more information on the structural organization of their nucleoli. The transformation of ring shaped nucleoli to nucleoli with more or less distinct nucleolonemata in PHA components in numbers indicating the activation of their production. This phenomenon seems to be related not only to the activation of the rRNA synthesis but also to its processing. The appearance of fibrillar RNP components in the central area of the ring shaped nucleoli apparently represents the 1st sign of the nucleolar RNA synthesis in these cells. The proportion of fibrillar and granular nucleolar RNP components in PHA unresponsive lymphocytes was similar to that in lymphocytes from patients with the usual type of lymphocytic leukemia. The intranucleolar chromatin areas appeared to be larger in PHA stimulated lymphocytes, but the proportion of these areas to the nucleolar body did not show substantial differences as compared to the resting cells.