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High-yield isolation of chloroplast DNA from Hordeum vulcare barley protoplasts

Karimov, M.; Lysenko, A.M.; Vinetskiĭ, I.P.

Biokhimiia 43(4): 597-601

1978

ISSN/ISBN: 0320-9725
PMID: 656491
Document Number: 133217
A fraction of intact chloroplasts free of other cell components in isolated from barley leaf chloroplasts. Instead of mechanical desintegration of plant tissue, the method described includes the cellulysine treatment, thus increasing the yeild of chloroplast DNA. The mean content of DNA per barley chloroplast is found to be 1.10(-4) g. Base composition of barley chloroplast DNA is 39.8 mol.% of G+C. The treatment of chloroplast DNA with restriction endonuclease EcoRI results in the appearance of 17-19 bands under agarose gel electrophoresis.

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