Effect of mutations in regulatory genes for alkaline phosphatase on the phosphohydrolase spectrum of E. coli periplasm
Maraeva, O.B.; Nesmeiapova, M.A.; Kulaev, I.S.
Biokhimiia 43(9): 1640-1647
1978
ISSN/ISBN: 0320-9725 PMID: 214171 Document Number: 131459
The isoform spectra of alkaline and acid phosphatase, pyrophosphataes, and ATPase in periplasm of E. coli were studied using electrophoresis in polyacrylamide gel with subsequent development of zimograms directly in the gel. Wild strains and mutants on 4 regulatory genes of alkaline phosphatase were analyzed. Mutations in regulatory genes were shown to influence the amount of each of the 3 isoforms of alkaline phosphatase and also the spectra of other phosphohydrolases.