Identification of Entamoeba histolytica membrane antigens with antibodies of amebiasis patients
Parkhouse, M.; Cid, M.E.; Calderon, J.
Archivos de Investigacion Medica 9(Suppl 1): 211-218
1978
ISSN/ISBN: 0066-6769 PMID: 211952 Document Number: 128932
The specificity of sera from patients with amoebiasis was assayed on trophozoites of E. histolytica HK9, HM15 and HM2 cultured axenically and monoxenically. Glutaraldehyde fixed cell were incubated with samples of serum, the excess of Igs was washed out and the presence of unbound material of specifically bound antibodies was measured with radiolabeled protein A from Staphylococcus aureus. A positive reaction with HK9 was observed in 18 per cent of the 66 sera tested. An immune crossreaction resulted with HM2 and HM15, while no reaction was observed with the nonpathogenic strains E. invadens, E. moshkovskii and E. laredo.