Effect of insulin on RNA and protein biosynthesis in liver mitochondria from normal and alloxan diabetic rats
Germanyuk, Y.L.; Minchenko, A.G.
Endocrinologia Experimentalis 12(4): 233-243
1978
ISSN/ISBN: 0013-7200 PMID: 312194 Document Number: 128173
In liver mitochondria from alloxan diabetic rats the biosynthesis of RNA (as measured by 14C-orotic acid incorporation and RNA polymerase activity) and protein (as measured by 14C-leucine incorporation) were decreased. In contrast, insulin (20 U kg-1) injected to intact or diabetic rats increased both these measures. However, no change of mitochondrial DNA biosynthesis (as measured by incorporation of 3H-thymidine) was found. It was concluded that in liver cells insulin plays an important role in regulation of biosynthesis not only of nuclear (cytoplasmic) but also of mitochondrial RNA and proteins.