Indirect immunoprecipitation by rat liver polyribosomes using antibodies to tyrosine aminotransferase
Chesnokov, V.N.; Blinova, N.N.; Mertvetsov, N.P.
Biokhimiia 43(4): 625-630
1978
ISSN/ISBN: 0320-9725 PMID: 26425 Document Number: 128077
A fraction of rat liver polyribosomes is isolated, which in its immunochemical characteristics considerably enriched with polyribosomes capable to synthesize hydrocortisone-induced liver tyrosine aminotransferase isoenzyme. This specific polyribosome fraction was purified by immunochemical fractionation of total liver polyribosomes using indirect precipitation. The content of polyribosomes in immunoprecipitates comprise 0.4-0.8% of its initial amount (before immunochemical fractionation). The ratio of specific polyribosomes in immunoprecipitates varies from 20 to 45%, which corresponds to 25-100-fold purification. The data obtained suggest that the method of indirect precipitation can be an efficient step in the isolation procedure of individual mRNA.