Effect of cortisol on the content of mRNA coding inducible tyrosine aminotransferase isoenzyme in rat liver
Mertvetsov, N.P.; Il'duganova, N.A.; Chesnokov, V.N.; Blinova, N.N.
Biokhimiia 43(6): 959-964
1978
ISSN/ISBN: 0320-9725 PMID: 27251 Document Number: 126541
In vitro estimation of synthesis of inducible tyrosine aminotransferase isoenzyme, directed by poly-A-containing RNA from liver of intact and corticol treated rats, is carried out. Total poly-A-containing RNA from liver polyribosomes of intact and induced rats was translated in cell-free system from wheat germs. Two antibodies immunoprecipitation was used to identify the translocation product (tyrosine aminotransferase). It was found that a synthesis of a specific protein product, precipitated by antibodies to tyrosine aminotransferase, takes place in cell-free system under translation of polysomic poly-A-containing liver RNA. The amount of immunoprecipitated product indicates, that the content of individual poly-A-containing mRNA for inducible tyrosine aminotransferase isoenzyme in liver of cortisol-induced rats is considerably higher than in intact animals.