Stationary kinetics of catalysis by the hydrogenase of Thiocapsa roseopersicina
Varfolomeev, S.D.; Gogotov, I.N.; Toaĭ, C.D.; Bachurin, S.O.
Molekuliarnaia Biologiia 12(1): 63-82
1978
ISSN/ISBN: 0026-8984 PMID: 24804 Document Number: 128058
Forward and reverse reactions of catalysis by a hydrogenase from T. roseopersicina in steady state have been investigated. The dependance of reaction rate on the concentration of substrates and of hydrogen ions has been studied. Detailed formal-kinetic analysis of possible mechanisms of enzyme action has been carried out and the classification of mechanisms of the reaction has been given. Comparison of experimental data with the equations obtained from formal-kinetic analysis has been undertaken and mechanisms compatible with experimental data were sorted out. Kinetic and molecular mechanism of the catalytic activity of hydrogenase has been proposed and elementary rate constants for the activation of molecular hydrogen have been calculated.