Properties of cholinesterase preparations from human erythroyctes
Brestkin, A.P.; Viaz'menskaia, M.M.; Dembo, M.A.; Maĭzel', E.B.; Raĭkher, I.I.
Voprosy Meditsinskoi Khimii 24(5): 608-612
1978
ISSN/ISBN: 0042-8809 PMID: 706252 Document Number: 127713
A modified method is described for isolation of acetylcholinesterase from human erythrocytes using an additional step of gel filtration on Sephadex G-75. Preparations of acetylcholinesterase were liberated from thromaboplastic activity and their specific activity was increased due to removal of low molecular proteins and of the products of destruction of hemoglobin. Content of A and B isoantigens in the preparations obtained was rather low and content of hemoglobin, combined with other proteins in the form of oxyhemoglobin, did not exceed 12% of the total protein.