Genetic characterization of the N protein of subgroups a and B human respiratory syncytial viruses
Zhang, Y.; Wang, H.-l.; Xie, Z.-d.; Kong, X.-h.; Liu, C.-y.; Shen, K.-l.; Xu, W.-b.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 23(2): 115-117
2009
ISSN/ISBN: 1003-9279 PMID: 20104752 Document Number: 12770
To clarify the genetic characteristics of N protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences. Reverse transciption polymerase chain reaction (RT-PCR) was performed to amplify the N protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in the year 2004. The RT-PCR products were sequenced for N protein coding region. The sequences of N protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed. The differences in number of nucleotide and deduced amino acid between 2 A Beijing 2004 isolates and prototype strain Long were 36-40 (3.1%-3.4%) and 4 (1.0%). The differences in number of nucleotide and deduced amino acid between 2 B Beijing 2004 isolates and prototype strain CH18537 were 17 (1.4%) and 1 (0.3%). The differences in number of nucleotide and deduced amino acid were 3-172 (0.25%-14.63%) and 0-18 (0-4.6%) respectively between 4 Beijing 2004 isolates and GenBank sequences. N gene is the highly conservative gene in the HRSV genome. The variation between A and B subgroups were widely distributed in the entire gene of N protein, while the variation within the A or B subgroups HRSV was considerably lower.
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