Correction of modal DNA values obtained by microspectrophotometry and tests for their shifts
Okagaki, T.; Izuo, M.
Journal of the National Cancer Institute 60(6): 1251-1258
1978
ISSN/ISBN: 0027-8874 PMID: 650696 Document Number: 126263
The technical problems in the application of statistical analyses of the nuclear DNA contents measured by plug or two-wavelength Feulgen microspectrophotometry were discussed. The skewness of the nuclear DNA content unimodally distributed in a cluster could be corrected by logarithmic transformation. A mean value of the logarithmically transformed DNA contents was a more logical characterization of cancer than a graphically obtained mode of nontransformed values. Equations were derived to estimate the number of cells in the G1- and S-phases of mitotic cycles contained within the cluster and to estimate the correction factor of the mean of transformed values of cells in the G1-phase. Thus statistical tests for a shift in the nuclear DNA content of the cells in question in the G1-phase from that of control cells were made possible.