Adrenocortical suppression & lysosomal stability
Philip, B.; Kurup, P.A.
Indian Journal of Biochemistry and Biophysics 15(3): 193-195
1978
ISSN/ISBN: 0301-1208 PMID: 748161 Document Number: 125781
Lysosomal stability was studied in rats in which adrenocortical insufficiency was induced by administration of aniline. The release of a typical lysosomal enzyme (.beta.-glucuronidase) was taken as a measure of lysosomal stability. Adrenocortical suppression resulted in a decrease in the activity of .beta.-glucuronidase in the intact lysosomes from the liver, while the soluble activity (released from the lysosomes) was increased. Total enzyme activity was not affected in the liver or in the aorta. Rate of release of .beta.-glucuronidase from lysosome-rich fraction from liver and aorta was also significantly more at various time intervals in the aniline-treated rats. The serum enzyme activity was also higher in these rats. These observations indicate that lysosomal stability is decreased on adrenocortical suppression. Cortisol in vitro restored the stability of the lysosomes from aniline-treated animals.