Top Abstract Purchase Preview Related
Home
>
Node
>
Subnode

Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for Light Cycler 1.2 instrument

Uttayamakul, S.; Likanonsakul, S.; Sutthent, R.; Chaovavanich, A.

Journal of the Medical Association of Thailand 90(11): 2429-2436

2007

ISSN/ISBN: 0125-2208
PMID: 18181331
Document Number: 12488
Commercial TaqMan real-time PCR reagent was modified and applied on Light Cylcer 1.2 for quantifying HIV-1 RNA in plasma and compared with the reference method; COBAS AmpliPrep/COBAS Amplicor HIV-1 monitor test version 1.5. Three hundred and eight frozen and fresh plasma samples were used for evaluation. Sequential specimens were also tested for follow-up cases. The correlation between HIV-1 RNA values obtained by reference and modified method with automated and manual sample preparation were significant with r = 0.916 and 0.908 (p < 0.001, p < 0.001) respectively with similar agreement log of mean bias (0.5 versus 0.48). High degree of correlation and agreement were observed between the assays in blind fresh plasma, r = 0.953 (p < 0.001) with 0.15 log difference in HIV-1 RNA level. Among follow-up samples, both methods gave 100% concordant results. This modified protocol provided evidence for using modified commercial real-time PCR reagent for HIV-1 RNA quantitative detection as a monitoring tool for HIV/AIDS patients in Thailand.

Document emailed within 1 workday
Secure & encrypted payments

Evaluation of HIV-1 viral load detection by modified a commercial real-time PCR reagent used for Light Cycler 1.2 instrument

Onyekwuluje, J.M.; Steinau, M.; Swan, D.C.; Unger, E.R. 2012: A Real-Time PCR Assay for HPV52 Detection and Viral Load Quantification Clinical Laboratory 58(1-2): 61-66
Supadej, K.; Intorasoot, S. 2012: Establishment of cheap and reliable real-time PCR for quantitation of HIV-1 viral load in plasma Journal of the Medical Association of Thailand 95(12): 1563-1568
Klausner, J.S.; Osborne, C.A.; Stevens, J.B. 1976: Clinical evaluation of commercial reagent strips for detection of significant bacteriuria in dogs and cats American Journal of Veterinary Research 37(6): 719-722
Mbida, A.é D.é; Sosso, S.; Flori, P.; Saoudin, H.; Lawrence, P.; Monny-Lobé, M.; Oyono, Y.; Ndzi, E.; Cappelli, G.; Lucht, F.éd.ér.; Pozzetto, B.; Oukem-Boyer, O.O.M.; Bourlet, T. 2009: Measure of viral load by using the Abbott Real-Time HIV-1 assay on dried blood and plasma spot specimens collected in 2 rural dispensaries in Cameroon Journal of Acquired Immune Deficiency Syndromes 52(1): 9-16
Fujimoto, T.; Shinohara, M.; Ito, M.; Okafuji, T.; Okafuji, T.; Nishio, O.; Yoshida, H.; Shimizu, H.; Chikahira, M.; Phan, G.T.; Ushijima, H. 2007: Detection of Dual-Infected Cases of Adenoviruses and Coxsackieviruses Type B by Real-Time PCR but not by the Conventional Viral Culture Technique Clinical Laboratory 53(9-10-11-12): 605-609
Ye, R.; Pan, J-cao.; Huang, Z-cheng.; Wang, H.; Wang, H-qiu.; Wei, D-fang.; Xu, K.; Wen, H-gen.; Chen, K-kai. 2005: Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction Zhonghua Yu Fang Yi Xue Za Zhi 39(2): 129-132
Wolff, D.; Gerritzen, A. 2011: Evaluation of a Real-Time PCR Assay for the Detection, Genotyping, and Quantification of Borrelia Burgdorferi Sensu Lato in Ixodes Ticks in a Routine Laboratory Setting Clinical Laboratory 57(1-2): 67-73
Watanaveeradej, V.; Sirirattanaphoomee, S.; Chantratita, W.; Nitayaphan, S.; Viputtikul, K.; Kerdpanich, A.; Samakoses, R.; Simasathien, S. 2005: Quantification of HIV-1 RNA load by one-tube-one-step RT PCR and real time PCR assay with TaqMan probe Journal of the Medical Association of Thailand 88(Suppl 3): S206-S213
Xie, J.-Z.; Chen, C.-R.; Zhang, J.; Ni, H.-Y.; Ge, S.-X.; Zhou, J.-J.; Ou, S.-H.; Zheng, X.-J.; Ran, P.; Pei, B. 2009: Quantitation of HTLV-i proviral load using real-time quantitative PCR with Taqman MGB probe Bing du Xue Bao 25(5): 339-343
Estill, J.; Aubrière, C.; Egger, M.; Johnson, L.; Wood, R.; Garone, D.; Gsponer, T.; Wandeler, G.; Boulle, A.; Davies, M.-A.; Hallett, T.B.; Keiser, O. 2012: Viral load monitoring of antiretroviral therapy, cohort viral load and HIV transmission in Southern Africa: a mathematical modelling analysis Aids 26(11): 1403-1413
Mekuria, L.A.; Prins, J.M.; Yalew, A.W.; Sprangers, M.A.G.; Nieuwkerk, P.T. 2016: Which adherence measure - self-report, clinician recorded or pharmacy refill - is best able to predict detectable viral load in a public ART programme without routine plasma viral load monitoring? Tropical Medicine and International Health: Tm and Ih 21(7): 856-869
Holguín, A.; Williams, L.; Castilla, J.; Soriano, V. 1997: Influence of time and storage conditions on plasma HIV viral load measurements Antiviral Therapy 2(4): 265-268
Chen, M.; Kim, J.H.; Kishida, N.; Nishimura, O.; Sudo, R. 2004: Enhanced nitrogen removal using C/N load adjustment and real-time control strategy in sequencing batch reactors for swine wastewater treatment Water Science and Technology: a Journal of the International Association on Water Pollution Research 49(5-6): 309-314
Hang, X.; Greenberg, N.L.; Shiota, T.; Firstenberg, M.S.; Thomas, J.D.; Thomas, J.D. 2000: Compression of real time volumetric echocardiographic data using modified SPIHT based on the three-dimensional wavelet packet transform Computers in Cardiology 27: 123-126
Dahouk, S.A.; Tomaso, H.; Karsten Nöckler; Neubauer, H. 2004: The Detection of Brucella spp. using PCR-ELISA and Real-Time PCR Assays Clinical Laboratory 50(7-8): 387-394
Jamjoom, M.B.; Azhar, E.A.; Tonkol, A-Kader.M.; Al-Harthi, S.A.; Ashankyty, I.M. 2006: Detection of malaria in Saudi Arabia by real-time PCR Journal of the Egyptian Society of Parasitology 36(3): 737-748
Jirous, J.; Saleh, A.; el-Tomi, N. 1984: The validity of real time scanning in detection of the cervical incompetence Sbornik Vedeckych Praci Lekarske Fakulty Karlovy University V Hradci Kralove 27(4): 589-596
Iudina, S.M.; Il'inskiĭ, I.A. 1984: Prognostic significance of the phenomenon of detection of modified effects in viral hepatitis B Sovetskaia Meditsina 12: 53-56
Simuyemba, M.C.; Ndlovu, O.; Moyo, F.; Kashinka, E.; Chompola, A.; Sinyangwe, A.; Masiye, F. 2020: Real-time evaluation pros and cons: Lessons from the Gavi Full Country Evaluation in Zambia Evaluation 26(3): 367-379
Calderaro, A.; Piccolo, G.; Gorrini, C.; Peruzzi, S.; Zerbini, L.; Bommezzadri, S.; Dettori, G.; Chezzi, C. 2006: Comparison between two real-time PCR assays and a nested-PCR for the detection of Toxoplasma gondii Acta Bio-Medica: Atenei Parmensis 77(2): 75-80