The induction of macrophage proliferation in vitro by a lymphocyte-produced factor
Hadden, J.W.; Sadlik, J.R.; Hadden, E.M.
Journal of Immunology 121(1): 231-238
1978
ISSN/ISBN: 0022-1767 PMID: 670701 Document Number: 124735
Sensitized guinea pig lymph node lymphocytes release on exposure to antigen a soluble mediator of 35-70,000 daltons, which induces proliferation of nonimmune oil-induced, monocyte-derived peritoneal and noninduced alveolar macrophages. Cell counts and nuclear labeling experiments indicate that this proliferation can be effectively assayed under conditions of monolayer culture by 3H thymidine incorporation. The magnitude and the kinetics of the proliferative response of macrophages in this system are dependent upon the concentration and the time of addition of the soluble mediator. Influences that operate to limit proliferation in this system appear to be high macrophage density, the production of prostaglandin by macrophages and other mediators present in the lymphokine preparation, such as MIF lymphocyte-mediated immunity, as occurs during infection with facultative intracellular bacterial pathogens.