Analysis of sponge sterols as the trimethylsilyl ethers and as the corresponding 5 alpha-and delta 4-3-ketosteroids using open-tubular gas chromatography-mass spectrometry. Application of selective enzymic oxidation

Edmonds, C.G.; Smith, A.G.; Brooks, C.J.; Edmonds, C.G; Smith, A.G; Brooks, C.J

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Analysis of sponge sterols as the trimethylsilyl ethers and as the corresponding 5 alpha-and delta 4-3-ketosteroids using open-tubular gas chromatography-mass spectrometry. Application of selective enzymic oxidation

Edmonds, C.G.; Smith, A.G.; Brooks, C.J.

Journal of Chromatography 133(2): 372-377

1977

ISSN/ISBN: 0021-9673

PMID: 838815

Document Number: 121961

Incubation with cholesterol oxidase which leads to conversion of the .DELTA.5- and 5.alpha.-3-.beta.-hydroxysteroids to .DELTA.4-and 5.alpha.-3-ketosteroids, respectively, easily separated on non-polar packed gas chromatography (GC) columns, and the resolution of .DELTA.5-and 5.alpha.-steroid pairs by GC on Silanox-type glass open-tubular columns were applied to the analysis of sterols from Hymeniacidon perleve and Grantia compressa. 5.alpha.-Cholestan-3.beta.-ol was the major constituent of both species; substantial proportions of cholesterol and 5.alpha.-cholest-7-en-3.beta.-ol were present in G. compressa; 17 other 4-demelthyl sterols were present in H. perleve.