Inhibition of polymorphonuclear leukocyte capping by a chemotactic factor
Mandell, B.F.; Spilberg, I.; Lichtman, J.
Journal of Immunology 118(4): 1375-1378
1977
ISSN/ISBN: 0022-1767 PMID: 321690 Document Number: 121861
Incubation of human polymorphonuclear leukocytes with colchicine and fluorescein-concanavalin A leads to the formation of a polarized cap of fluorescence not seen if cells are incubated with fluorescein-Con A along. When cells are preincubated with a chemotactic factor before colchicine treatment, the capping is inhibited in a dose-related manner. Studies with alpha-methylmannoside indicate that the caps represent extracellular fluorescein-Con A and are not areas of Con A internalization. Experiments utilizing an irreversible inhibitor of serine esterases suggest that a chemotactic factor-activated enzyme is involved in the inhibition of cap formation in the human neutrophil.