The role of RNA metabolism in polymorphonuclear leukocyte phagocytosis
Kasprisin, D.O.; Harris, M.B.
Journal of Laboratory and Clinical Medicine 90(1): 118-124
1977
ISSN/ISBN: 0022-2143 PMID: 874363 Document Number: 113660
The role of RNA metabolism in human PMN phagocytosis was examined. By the inhibition of RNA synthesis with actinomycin D, intracellular killing of a strain of actinomycin D-resistant Staphylococcus epidermidis was unaffected by 1 h but significantly decreased by 2 and 3 h. After 1 h, phagocytosis began to decrease, and iodination of proteins also decreased in the presence of actinomycin D. Hexosemonophosphate shunt activity remained similar for both actinomycin D treated PMN and control cells at 30 min and 2 h. Protein synthesis was inhibited by incubating PMN with puromycin. The ability of PMN to kill puromycin-resistant S. epidermidis was significantly decreased at 2 and 3 h.