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Determination of phagocytosis of 32P-labeled Staphylococcus aureus by bovine polymorphonuclear leukocytes

Dulin, A.M.; Paape, M.J.; Weinland, B.T.

American Journal of Veterinary Research 45(4): 786-789

1984

ISSN/ISBN: 0002-9645
PMID: 6731995
Document Number: 241630
A procedure for the measurement of phagocytosis by bovine polymorphonuclear leukocytes (PMN) of 32P-labelled S. aureus was modified so that a larger number of samples could be compared in a single run, and smaller volumes of sample, PMN, and 32P-labelled S. aureus could be used. Results were highly reproducible, with a coefficient of variation between duplicate determinations of 2%. Lysostaphin was prepared from the supernatant of S. staphylolyticus and was compared with a commercially available preparation. Effects of lysostaphin on PMN and influence of incubation media on release of 32P from 32P-labelled S. aureus by lysostaphin were examined.

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