Detection of the intra-organ microcirculatory bed by the calcium-adenosine triphosphate and staining methods
Chilingarian, A.M.
Zhurnal Eksperimental'noi i Klinicheskoi Meditsiny 17(5): 19-28
1977
ISSN/ISBN: 0514-7484 PMID: 75624 Document Number: 121504
A method was developed for Ca-ATP exposure of the intraorgan microcirculatory bed based on the selective precipitation of Pi, separated from ATP by Ca2+. Subsequently the product of the reaction transformed into a black lead sulfide. The method produced precise contrast exposure of the vascular-capillary network in the majority of cat organs in thick sections and membranes. A significant advantage of the method was the capacity for simultaneous and differentiating study of venous and arterial beds. Arteries stood out in preparations due to coloration of their smooth-muscle cells.