A sulphated glycopeptide in human supragingival calculus extracts
Embery, G.
Calcified Tissue Research 23(1): 13-17
1977
ISSN/ISBN: 0008-0594 PMID: 407987 Document Number: 121057
A method is described for the isolation and purification of a sulfated glycopeptide from human supragingival calculus. The compound was isolated after using EDTA treatment, 2 M CaCl2 extraction, proteolytic digestion, ethanol precipitation, and finally purified by DEAE cellulose chromatography. It migrated as a single component on cellulose acetate electrophoresis, and chemical and IR spectral analysis showed the presence of covalently attached sulfate groups. The sulfated glycopeptide was distinguished from a sulfated glycosaminoglycan.