The effect of riboflavin deficiency on white cell glutathione reductase in rats
Müller, E.M.; Bates, C.J.
International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift für Vitamin- und Ernahrungsforschung. Journal International de Vitaminologie et de Nutrition 47(1): 46-51
1977
ISSN/ISBN: 0300-9831 PMID: 844948 Document Number: 120289
The measurement of glutathione reductase in white cells from peripheral rat blood is described; the contribution from red cell glutathione reductase was virtually eliminated. The activation of the white cell enzyme by FAD in vitro was measured during an 8 wk period of dietary riboflavin deficiency in weanling rats. The activation increased progressively during the deficiency reaching about 50% after 8 wk, at which time liver FAD levels had declined to 30% of the control levels and clinical symptoms of deficiency were beginning to appear. No increase in activation of white cell glutathione reductase was observed in pair-fed controls, over this time period. The measurement of the activation coefficient of white cell glutathione reductase could usefully supplement that of the red cell enzyme, in defining riboflavin status.