Determination of Oxygen Derived Free Radicals Producer (Xanthine oxidase) and Scavenger (Paraoxonase1) Enzymes and Lipid Parameters in Different Cancer Patients
Samra, Z.Q.; Pervaiz, S.; Shaheen, S.; Dar, N.; Athar, M.A.
Clinical Laboratory 57(9-10): 741-747
2011
ISSN/ISBN: 1433-6510 PMID: 22029190 Document Number: 11982
Background: Cancer is a major cause of death throughout the world. Xanthine oxidase (XO) is considered an oxygen derived free radicals producer and paraoxonase1 (PON1) is known as a free radicals scavenger. This study was undertaken to determine the activity of PON1 and XO and their concentration along with the lipid profile, lipid peroxide, and thiol level in cancer patients and healthy persons. Methods: Antigen competitive ELISA was used to determine the level of paraoxonase1 and xanthine oxidase in plasma. Paraoxonase and arylesterase activity of the PON1 enzyme and the activity of the XO enzyme were also determined. PON1 and XO activity, cholesterol, LDL-C, HDL-C, lipid peroxides, and thiol level in patients with breast cancer (n = 50), prostate cancer (n = 20), lung cancer (n = 12,), cervix cancer (n = 15), Non-Hodgkin lymphoma (n = 10), and acute lymphoblastic lymphoma (n = 8) and total age matched healthy persons (n = 115) were studied. Results: Low plasma paraoxonase/arylesterase activities (p 0.05) were observed in cancer patients. Conclusions: : Lower PON1 and high XO enzyme activities cause an imbalance of the free radical system which enhances the lipid peroxidation and other pathological conditions. Lower HDL-C level is also indicative of the low level of PON1 enzyme activity.
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