Detection of inducible clindamycin resistance in staphylococci by disk diffusion induction test
Chelae, S.; Laohaprertthisarn, V.; Phengmak, M.; Kongmuang, U.; Kalnauwakul, S.
Journal of the Medical Association of Thailand 92(7): 947-951
2009
ISSN/ISBN: 0125-2208 PMID: 19626815 Document Number: 11972
To detect inducible clindamycin (CL) resistance in staphylococci by disk diffusion induction test (D-test). One thousand one hundred eighty clinical isolates of staphylococci were tested for inducible CL resistance by placing erythromycin (E) disk and clindamycin disk 12 mm apart (edge to edge) on Mueller-Hinton agar plate inoculated with staphylococci. The flattening of CL zone (D-shaped zone) near E disk indicated an inducible CL resistance was observed after 18-24 h of incubation. Inducible CL resistance was detected in 9.9% of staphylococci isolates. It was found in methicillin-resistant Staphylococcus aureus (MRSA) more than methicillin-sensitive Staphylococcus aureus (MSSA) and coagulase-negative staphylococci (CoNS) 35.9%, 4.7%, and 5.5%, respectively. To avoid misinterpretation of CL result, D-test is recommended for routine detecting of inducible CL resistance in staphylococci. It provides the confident laboratory report of CL as resistant (D-shaped zone positive) or as susceptible (D-shaped zone negative) particular for E resistant isolates.
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