Comparison of Methods of Detecting Bacterial Biofilm

Tajima, Y.; Yamaguchi, T.; Takagi, R.; Nakajima, T.; Kominato, Y.

Clinical Laboratory 56(3-4): 143-147

2010


ISSN/ISBN: 1433-6510
PMID: 20476646
Document Number: 11693
Fresh bacterial biofilm produced by Staphylococcus epidermidis was sensitively detected by using a chemiluminescent probe. Its lower detection limit was around 10 -5 - 10 -4 dilution (equivalent to 10 -5 - 10 -4 cm² biofilm) - 10² - 10 3 times as sensitive as ordinary colorimetric methods. The linearity and dynamic range were slightly better than those of fluorometry.

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Comparison of Methods of Detecting Bacterial Biofilm