Evaluation and improvement of different methods for the enrichment of antigen-binding cells of chickens
Gattringer, C.; Wolf, H.; Wick, G.
Folia Biologica 23(6): 432-435
1977
ISSN/ISBN: 0015-5500 PMID: 608487 Document Number: 116617
Antigen-binding cells were enriched using bovine serum albumin immunized chickens as an experimental model. A comparison of density centrifugation, adherence to derivatized surfaces (polystyrol and gelatin) and affinity chromatography revealed that optimal enrichment was obtained using the latter procedure. Polyacrylamide was superior to agarose.
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