Proposals for quality control methods of bacterial vaccines for immunostimulation. II. in vivo and in vitro stimulation of lymphocytes by BCG
Bruynzeel, D.P.; Ettekoven, H.; Kreeftenberg, J.G.
Developments in Biological Standardization 38: 91-96
1977
ISSN/ISBN: 0301-5149 PMID: 608553 Document Number: 114246
An in vitro test for measuring the reaction of the draining lymph node after BCG vaccination and an in vitro test based on the stimulation of lymphocytes by concanavalin A were used to demonstrate immunostimulating properties of different BCG vaccines. A marked difference was seen between vaccines grown as a surface pellicle and ground in a ball mill, and those which were prepared from dispersed homogeneous cultures. In both systems the ball mill preparations were the less active.