Solvent effects on halothane: 19F nuclear magnetic resonance in solvents and artificial membranes
Koehler, L.S.; Cruley, W.; Koehler, K.A.
Molecular Pharmacology 13(1): 113-121
1977
ISSN/ISBN: 0026-895X PMID: 834180 Document Number: 114195
This study of solvent effects on halothane 19F NMR chemical shifts was undertaken in order to elucidate environmental factors contributing to observed halothane 19F NMR shifts in the presence of phospholipid suspensions in aqueous media. Halothane 19F NMR chemical shifts are correlated by Hildebrand's .delta. function or refractive index for non-H-bonding solvents. No significant correlation was observed for other solvent properties, such as dielectric constant. Water and alcohols show positive deviations from the correlation with .delta. Halothane molecules associated with synthetic and natural phospholipid multilayer vesicles show significant line broadening of the F signal and appear to be in an environment which may be characterized by a .delta. value of 9.3 .+-. 0.3 (cal/cm3)1/2 by comparison with the observed correlation of chemical shift with .delta. in isotropic solvent systems.