Gnotobiotic calves: derivation, maintenance, Thiry-Vella loop preparation, and ethylene oxide gas sterilization
Newman, L.E.; Whitehair, C.K.; Mullaney, T.P.
American Journal of Veterinary Research 47(12): 2632-2636
1986
ISSN/ISBN: 0002-9645 PMID: 3800124 Document Number: 113
Twenty-four pregnant Holstein cows were used to develop techniques and procedures to obtain calves by hysterotomy and to maintain them in a closed gnotobiotic environment (sterile plastic-film isolators) for as long as 10 days for infectious disease research. Thiry-Vella loop preparation for obtaining intestinal loop secretions was done in a surgical isolator and was successful in 12 of 14 gnotobiotic calves. Ethylene oxide gas was effective in sterilizing heat-labile and moisture-sensitive instruments and equipment.
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