Autologous Fibrin-Base Scaffold for Chondrocytes and Bone Marrow Mesenchymal Stem Cells Implantation: The Development and Comparison to Conventional Fibrin Glue
Kasemkijwattana, C.; Rungsinaporn, V.; Siripisitsak, T.; Kongmalai, P.; Boonprasert, R.; Charoenthamruksa, C.; Hongeng, S.; Wongkajornsilp, A.; Muangsomboon, S.; Chaipinyo, K.; Chansiri, K.
Journal of the Medical Association of Thailand 99 Suppl. 8: S99
2016
ISSN/ISBN: 0125-2208 PMID: 29901946 Document Number: 11094
The authors developed the autologous fibrin-base scaffold for chondrocytes and bone marrow mesenchymal stem cells (BM-MSCs) implantation and evaluated cells viability in autologous fibrin-base scaffold comparing to commercial fibrin glue. The chondrocytes and BM-MSCs were seeded into autologous fibrin-base scaffold and commercial The chondrocytes and BM-MSCs could not survive in commercial fibrin glue. The chondrocytes and BM-MSCs in autologous fibrin-base scaffold could proliferate and synthesize the cartilaginous matrices on Alcian blue, Saffanin-0, Toluidine blue, and Collagen type II staining at 6 weeks. The fixation strength is excellent. The developed autologous fibrin-base scaffold can be used as the scaffold for chondrocytes and BM-MSCs