RNA-synthesizing complex of the Sendai virus in the nucleoli of infected Erlich ascitic carcinoma cells
Agafonova, L.V.; Vladimirtseva, E.A.; Bukrinskaia, A.G.
Voprosy Virusologii 5: 544-551
1977
ISSN/ISBN: 0507-4088 PMID: 201099 Document Number: 110248
A virus-specific complex with sedimentation constant of 250S and buoyant density 1.35 g/cm3 in cesium chloride density gradient is formed within 20--24 hours after infection in nucleoli of Ehrlich ascitic carcinoma cells infected with Sendai virus. The complex contains a rapidly sedimenting RNA (70--90S) which is about 50% stable to RN-ase. The complex has RNA-polymerase activity in a cell-free system; the product of the RNA-polymerase reaction is a RNA with sedimentation constant 50S. It is suggested that the virus-specific structure with sedimentation constant 250 S found in nucleoli is the Sendai virus replicative complex.