Isolation of phospholipase D from cotton seeds
Mad'iarov, S.R.
Biokhimiia 41(2): 255-259
1976
ISSN/ISBN: 0320-9725 PMID: 1276267 Document Number: 109968
A method of isolating homogenous phospholipase D from cotton seeds is described. The homogeneity of the enzyme preparation is demonstrated by means of polyacrylamide disc electrophoresis, gel filtration through Sephadex G-200 and determination of N-terminal amino acid residue, which turned to be glutamic acid. Molecular weight of the enzyme is found to be 71000 +/- 3000. Several equilibrium forms of the enzyme are observed.
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