Effects of adriamycin on the reverse transcriptase and the production of murine leukemia virus
Tomita, Y.; Kuwata, T.
Cancer Research 36(9 Pt.1): 3016-3019
1976
ISSN/ISBN: 0008-5472 PMID: 61807 Document Number: 107826
Adriamycin inhibited the endogenous RNA-, poly(A) .cntdot. d(T)12- and calf thymus DNA catalyzed reaction of reverse transcriptase from AKR mouse murine leukemia virus (AKR-MLV). This inhibition was found at the reaction levels of endogenous RNA-directed and subsequent DNA-directed DNA synthesis. Although adriamycin and actinomycin D significantly reduced the growth of AKR mouse cells (K3b), treatment with adriamycin could not inhibit AKR-MLV production in these cells. Actinomycin D inhibited AKR-MLV production completely in the same experimental condition. In adriamycin resistant K3b/Am cells, which were isolated by intermittent treatment of K3b cells with adriamycin, persistence of AKR-MLV was demonstrated. K3b/Am cells showed some altered characteristics such as reduced growth rate and tumorigenicity.