Localization of the Meler's reaction with ethanol catalase trap in the chain of photosynthetic electron transport
Bekina, R.M.; Lebedeva, A.F.; Rubin, B.A.
Biokhimiia 41(5): 815-821
1976
ISSN/ISBN: 0320-9725 PMID: 1030637 Document Number: 107295
The common view of photosystem I as the action site of catalase and ethanol at oxygen uptake in chloroplasts are based on indirect data on this reaction. That is why the question on Mehler reaction localization in electron transport chain with ethanolcatalase trap has been investigated anew. It has been demonstrated that oxygen uptake with catalase and ethanol does not decrease in presence of dibromothymoquinone (2,5-dibromo-3-methyl-6 isopropyl-p-benzoquinone--DBTQ) which blocks electron transfer to photosystem I at plastoquinones level. The summation of oxygen uptake activities is observed on the combined action of catalase and ethanol with any of the Mehler reagents functioning in photosystem I (methylviologen,FMN, epinephrine, ferredoxin). Catalase and ethanol in contrast to methylviologen have no effect on photooxidation rate of reduced dichlorphenolindophenol in photosystem I. The quatum yield of oxygen uptake with catalase and ethanol versus wave length of actinic light shows a distinct maximum in the photosystem II absorption area and a "red drop" in the longware area. The obtained data show that the Mehler reaction with catalase and ethanol takes place in photosystem II only.