Effect of feeding on catabolism and activity of exogenous gastrin in dogs
Woussen-Colle, M.C.; Limbosch, J.M.; Keuppens, F.; de Graef, J.
Acta Hepato-Gastroenterologica 23(3): 216-221
1976
ISSN/ISBN: 0300-970X PMID: 941625 Document Number: 107067
Four of 8 dogs weighing 12 to 18 kg with vagally denervated Heidenhain pouch of the oxyntic gland area were antrectomized. The dogs were starved for 24 h before they were given by vein isotonic saline, heparin, synthetic human gastrin, 3 mu g/kg h, pig gastrin, 4 mu g/kg h, or histamine hydrochloride, 0.08 mg/kg h. A meal of 250 g boiled sheep liver was given to the dogs 75 min after the start of gastrin infusion. In control tests the dogs got the gastrin infusion without the meal or the meal without gastrin or histamine infusion. Gastric juice was collected every 15 min for estimation of acidity. Blood was sampled for measurement of serum gastrin. In all the dogs given gastrin, feeding decreased serum gastrin by about 15 to 20% 60 min after feeding; the gastrin concentration returned to the control value within 3 h after feeding. In the non-antrectomized dogs, infusion of a near maximal dose of gastrin increased the acid response to the meal above the maximal histamine acid output. Exogenous gastrin also increased the acid response to a meal in antrectomized dogs. The conclusion was that feeding increases the metabolic clearance rate of exogenous gastrin, an effect which could be at least partly related to changes in the intestinal blood flow; and that, after feeding, one or several hormones capable of stimulating acid secretion but unrelated to gastrin are probably released.