Recommendations for the description of species and biotypes of the genus Brucella
Morgan, W.J.; Corbel, M.J.
Developments in Biological Standardization 31: 27-37
1976
ISSN/ISBN: 0301-5149 PMID: 1261743 Document Number: 106896
The following criteria are given as a basis for classification of an organism in Brucella: Gram-negative coccobacillary morphology; a G +C content for the DNA of 56-58 moles%; a minimum of 90% homology with the DNA of reference strains in hybridization tests; disk electrophoretograms of acid-phenol soluble proteins of identical pattern to reference strains; a cytochrome C absorption spectrum with absorption maxima between 522-530 nm and 552-560 nm; extensive serological cross-reactions of intracellular antigens with those of reference strains. Supporting evidence is given. Subdivision into species depends on principal natural host, oxidative metabolic pattern and phage sensitivity. Classification into biotypes depends on CO2 requirement, H2S production, dye sensitivity and reaction with monospecific antiserum to A and M antigens.