Localization of pyruvic acid in the histidine decarboxylase of Micrococcus sp. n
Alekseeva, A.E.; Prozorovskiĭ, V.N.
Biokhimiia 41(9): 1584-1587
1976
ISSN/ISBN: 0320-9725 PMID: 974173 Document Number: 106759
A fragment containing the pyruvic acid residue is isolated from hymotryptic hydrolyzate of alpha-polypeptide chain of carboxymethylated histidine decarboxylase (HD) 14C-phenylhydrasone. The pyruvate residue is estimated to be bind with alpha-amino group of N-terminal phenylalanine in alpha-polypeptide chain. N-terminal alanine is found in HD alpha-polypeptide chain after its reductive amination. It makes possible to determine the estimation of N-terminal amino acid sequence in HD alpha-chain.