Accurate amniotic fluid bilirubin analysis from the "bloody tap". A preliminary report
Hochberg, C.J.; Witheiler, A.P.; Cook, H.
American Journal of Obstetrics and Gynecology 126(5): 531-534
1976
ISSN/ISBN: 0002-9378 PMID: 984122 Document Number: 106711
The quantitation of bilirubin in amniotic fluid is of paramount importance in prognosticating the severity of Rh isoimmunization. Amniotic fluid contaminated by maternal blood can result in erroneous results when direct spectrophotemetric analysis is performed on such fluid. The technique and results of a one-step chloroform extraction performed on "bloody" amniotic fluid is presented. Results confirm that chloroform extraction yields more accurate clinical information in regard to Rh isoimmunized patients in whom a "bloody tap" is obtained.