Spectrophotometric analysis of amniotic fluid in Rh immunised pregnancies
Gupta, I.; Mahajan, U.; Sawhney, H.; Jolly, J.G.
Indian Journal of Medical Research 92: 24-27
1990
ISSN/ISBN: 0019-5340 PMID: 2112116 Document Number: 356901
A total of 307 amniotic fluid analysis done in 344 Rh negative immunised mothers showed that 46, 83 and 178 .DELTA. OD values at 450 m.mu. fell in the upper, middle and lower zones of Liley's charts respectively. The correlation of spectrophotometric analysis with the condition of the baby at birth was about 95 per cent in the upper and lower zones. In the middle zone, however, it was about 75 per cent only. Also, in 7 women in whom the OD at 450 m.mu. fell in the middle zone, the babies were found to be Rh negative; in another baby, the OD differnce fell in upper zone. In spite of these limitations amniotic fluid examination seems to be an important single guide to severity, being superior to other parameters like previous obstetric history, antibody titre alone and Liley's charts, which are still widely used.