Kinetics and morphology of immune reactve cells in the spleen as observed uwing the immune adherence method. III. Spontaneous immunocytoadherence as influenced by total body irradiation
Blazek, J.
Ceskoslovenska Patologie 12(2): 83-88
1976
ISSN/ISBN: 1210-7875 PMID: 1087582 Document Number: 106667
The course of spontaneous immunocytoadherence as influenced by a total body 850 r irradiation was studied in the mouse spleen. Prior to the total body irradiation 0.87 RFC/100 cells occurring spontaneously, without any previous antigenic stimulus, were dislosed in the spleen. Twenty four hours after iraadiation 0.075 RFC/1000 cells were detected. This number dropped to 0.023 RFC/1000 cells at day 4 after irradiation. A qualitative morphological analysis showed the following values: 65,1% of the spontaneous RFC were small lymphocytes, 24.5% were medium-sized lymphocytes and 3% large lymphocytes; 1.1% were plasma cells and 5.4% macrophages. Twenty four hours after irradiation with a dose of 850 r the RFC contained 9.3% plasma cells, and 90.7% macrophages, the respective numbers on day 2 being 7.5% and 98.3%. At day 4, permitting to demonstrate the RFC in significant numbers there occurred macrophages only. The model experiment has shown that the formation of spontaneous rosettes represents a cirtually normal immune reaction.