Production and characteristics of glycerin mutants of S. typhimurium
Ravdonikas, L.E.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 12: 29-32
1976
ISSN/ISBN: 0372-9311 PMID: 799451 Document Number: 106487
S. typhimurium glycerine mutants-gly 90 and gly 87 were obtained under the action of ethylmethanesulfonate. The mutants obtained were characterized by the site of block in the course of glycerine utilization:gly 90 mutant bore the mutation in the glp K-gene, this leading to the defect by glycerokinase-the enzyme of the first stage of glycerine metabolism; gly 87 mutant had derangements in the other stage of glycerine utilization. Both mutants lost their capacity to induce keratoconjunctivitis in guinea pigs, but differed by the LD50 value in intraperitoneal infection of albino mice (y gly 87--5 cells, y gly 90--1x10(7) cells).