Association of Cholinergic Muscarinic 2 Receptor Gene Polymorphisms with Learning Aptitude among Medical and Fine Arts Students
Thongket, P.; Pleansiri, C.; Thurakitwannakarn, W.; Sukhumsirichart, W.
Journal of the Medical Association of Thailand 99 Suppl. 8: S201-S205
2016
ISSN/ISBN: 0125-2208 PMID: 29906045 Document Number: 10401
Cholinergic muscarinic 2 receptor (CHRM2) is believed to be involved in neuronal excitability, synaptic To investigate single nucleotide polymorphisms (SNPs) in CHRM2 gene with the learning aptitude among medical and fine arts students at Srinakharinwirot university, Thailand. A total of two hundred blood samples were withdrawn from medical (100) and fine arts (100) students. DNAs were extracted using DNA extraction kit. SNP primers were designed and screened. Genotyping was performed by real-time PCR and high-resolution melting (HRM) analysis and confirmed by sequencing. The difference in genotypic distribution was analyzed using Pearson's Chi-square test implemented in SPSS program version 11.5. Significant level was set at p<0.05. Two SNPs in CHRM2 gene, rs2061174 and rs6948054, showed significant difference in genotype distribution between medical and fine arts students (p<0.05). The rs2061174 showed significant at p = 0.001, OR and 95% CI were 3.78 (2.00-7.14), whereas the rs6948054 was significant at p = 0.012, OR and 95% CI were 2.50 (1.32-4.77). Two SNPs in CHRM2 gene, rs2061174 and rs6948054, may be used as biomarker to distinguish the learning aptitude among Thai individual.