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Synthesis and characteristics of proteins in inner mitochondrial membranes of the myocardium of rats with disseminated necrotic heart diseases

Kopteva, L.A.; Zhevoliukova, L.I.; Grigor'eva, E.A.

Biulleten' Eksperimental'noi Biologii i Meditsiny 80(12): 90-93

1975

ISSN/ISBN: 0365-9615
PMID: 1225409
Document Number: 94952
Two fractions of the inner membranes of the mitochondria were isolated from rat myocardium: complex-the inner membranes-matrix (fraction A); the aggregates of the inner membranes deproved of the matrix (fraction B). Both fractions of the membranes effectuated the incorporation of the radioactive amino acids with the same activity. With the aid of gel electrophoresis the amount of the protein components of A and B fractions was found to bary from 20 to 30. The mol wts were from 10000 to 200000. Disseminated myocardial necrosis produced by novodrine injection based on the ischemic state of the heart led in two days to a considerable decrease in the protein synthesizing activity of fractions A and B. The protein profiles and the mol wt of the membrane protein components displayed no marked changes.

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