Microassay of an arylamidase in needle biopsies of human liver
Sato, T.; Mori, K.; Ito, S.
Research Communications in Chemical Pathology and Pharmacology 11(2): 257-264
1975
ISSN/ISBN: 0034-5164 PMID: 1153871 Document Number: 92500
A method is described for determination of liver arylamidase activity in homogenates of the small amount of human as well as experimental animal tissues (approx. 5-15 mg wet wt.) obtained by needle biopsy. One milligram of human liver contains 90-130 mug protein. The arylamidase activity in normal liver was 0.2 to 0.6 nmoles benzylhydrazine formed/mug protein/hr. The microdetermination of an arylamidase described in this paper is worth in the diagnosis of hepatic injury because of the use of biopsy sample.