An in vitro fibroblast-enriched sponge preparation for induction of intrauterine adhesions
Schenker, J.G.; Nicosia, S.V.; Polishuk, W.Z.; Garcia, C.R.
Israel Journal of Medical Sciences 11(8): 849-851
1975
ISSN/ISBN: 0021-2180 PMID: 1165182 Document Number: 90149
Induction of intrauterine adhesions may be of significant clinical importance for treatment of dysfunctional bleeding and as a method of contraception. A method which was successfully used to seed sponge matrices with fibroblasts was described; this may be used to induce intrauterine adhesions by s.c. implantation. Collagen-coated cellulose sponges were fed with 3T6 mouse fibroblasts, a collagen-secreting cell line. The sponges were observed daily by phase contrast microscopy; sponges with fibroblasts were fixed in Bouin's fixative and processed for conventional light microscopy. Examination of sections stained with hematoxylin and eosin showed a large number of fibroblasts growing in various directions within the spaces of the sponges. Growth seemed to occur preferentially along the collagen layer, and small nodular cell colonies were seen in a few areas.