A purine nucleoside hydrolase from Trypanosoma gambiense, purification and properties
Schmidt, G.; Walter, R.D.; Königk, E.
Tropenmedizin und Parasitologie 26(1): 19-26
1975
ISSN/ISBN: 0303-4208 PMID: 238316 Document Number: 83534
A purine nucleoside hydrolase from Trypanosoma gambiense was purified 160-fold. Preferred substrates of the reaction were adenosine, inosine and guanosine with a maximum of activity at pH 5.4. Competitive inhibitors of the adenosine hydrolysis were dimethylallyl adenosine, 6-methylmercaptopurine riboside, tubercidin, formycin B, 6-mercaptopurine riboside and deoxyadenosine. A metabolic scheme of adenosine nomophosphate salvage synthesis is discussed.